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SV388 is a notable chicken embryo fibroblast cell line that was originally derived from the embryonic tissues of the White Leghorn chicken breed. Established in the late 20th century, SV388 has gained significant attention in the fields of virology, molecular biology, and vaccine development due to its unique biological properties and versatility in laboratory research.
Origin and Characteristics
SV388 was developed in the laboratory of Dr. H. Ian Freshney during research focused on avian viruses. The cell line is characterized by its diploid nature, which allows it to maintain a stable karyotype under standard culture conditions. This stability is crucial for many experimental protocols, particularly those involving the study of viral pathogenesis and host-pathogen interactions. SV388 exhibits a fibroblast-like morphology, with elongated and spindle-shaped cells that grow adherently in culture dishes. Moreover, it has a relatively high growth rate, making it an ideal candidate for various laboratory applications.
Applications in Research
One of the primary applications of SV388 is in virology, particularly for studying avian diseases. The cell line is permissive to several avian viruses, including the Marek’s disease virus (MDV) and various strains of the avian influenza virus. Researchers utilize SV388 to investigate viral replication, pathogenesis, and the host's immune response. Its use has greatly contributed to the understanding of these pathogens and has aided in the development of effective vaccines.
Additionally, SV388 is used in cancer research. Its fibroblast nature allows scientists to delve into tumor biology, including transformation processes, cell proliferation, and the effects of various oncogenes. Researchers often utilize SV388 to study the interactions between transformed cells and their microenvironment, providing insights into cancer progression and metastasis.
Furthermore, SV388 is employed in studies relating to cell signaling, gene expression, and pharmacological testing. The cell line serves as a model system to investigate the effects of various compounds on cell behavior, which is essential for drug discovery and development processes.
Preservation and Distribution
The availability and preservation of SV388 have been facilitated by various biological resource centers, url which provide researchers with access to this invaluable cell line. Proper protocols for freezing and thawing SV388 ensure that researchers can maintain its viability and characteristics over extended periods, allowing for reproducibility in experiments.
Conclusion
In summary, SV388 is a fundamental chicken cell line that has proven to be a versatile tool in various areas of biological research. Its applications in virology, cancer research, and pharmacology exemplify its significance within the scientific community. As researchers continue to explore novel applications and improve cell culture techniques, SV388 will undoubtedly remain an essential component in understanding avian biology, viral interactions, and potential therapeutic strategies. Its ongoing use reinforces the importance of avian models in biological research and highlights the contributions of SV388 to advancements in biomedical sciences.
Origin and Characteristics
SV388 was developed in the laboratory of Dr. H. Ian Freshney during research focused on avian viruses. The cell line is characterized by its diploid nature, which allows it to maintain a stable karyotype under standard culture conditions. This stability is crucial for many experimental protocols, particularly those involving the study of viral pathogenesis and host-pathogen interactions. SV388 exhibits a fibroblast-like morphology, with elongated and spindle-shaped cells that grow adherently in culture dishes. Moreover, it has a relatively high growth rate, making it an ideal candidate for various laboratory applications.
Applications in Research
One of the primary applications of SV388 is in virology, particularly for studying avian diseases. The cell line is permissive to several avian viruses, including the Marek’s disease virus (MDV) and various strains of the avian influenza virus. Researchers utilize SV388 to investigate viral replication, pathogenesis, and the host's immune response. Its use has greatly contributed to the understanding of these pathogens and has aided in the development of effective vaccines.
Additionally, SV388 is used in cancer research. Its fibroblast nature allows scientists to delve into tumor biology, including transformation processes, cell proliferation, and the effects of various oncogenes. Researchers often utilize SV388 to study the interactions between transformed cells and their microenvironment, providing insights into cancer progression and metastasis.
Furthermore, SV388 is employed in studies relating to cell signaling, gene expression, and pharmacological testing. The cell line serves as a model system to investigate the effects of various compounds on cell behavior, which is essential for drug discovery and development processes.
Preservation and Distribution
The availability and preservation of SV388 have been facilitated by various biological resource centers, url which provide researchers with access to this invaluable cell line. Proper protocols for freezing and thawing SV388 ensure that researchers can maintain its viability and characteristics over extended periods, allowing for reproducibility in experiments.
Conclusion
In summary, SV388 is a fundamental chicken cell line that has proven to be a versatile tool in various areas of biological research. Its applications in virology, cancer research, and pharmacology exemplify its significance within the scientific community. As researchers continue to explore novel applications and improve cell culture techniques, SV388 will undoubtedly remain an essential component in understanding avian biology, viral interactions, and potential therapeutic strategies. Its ongoing use reinforces the importance of avian models in biological research and highlights the contributions of SV388 to advancements in biomedical sciences.
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